STUDY ON INHIBITORY EFFECT OF ROSMARINUS OFFICINALIS L EXTRACTS AND QUERCITINE ON PARTIALLY PURIFIED COW’S BRAIN POLYAMINE OXIDASE
- Title
- STUDY ON INHIBITORY EFFECT OF ROSMARINUS OFFICINALIS L EXTRACTS AND QUERCITINE ON PARTIALLY PURIFIED COW’S BRAIN POLYAMINE OXIDASE
- Abstract
-
This study involved the recognition of polyamine oxidase PAO in cow’s brain extract, using dialysis and DEAEcellulose ion exchanger chromatography. The enzyme was partially isolated and the purification fold of just onepeak (I) obtained
was 17.71. Rosemary (Rosmarinus officinalis L.) for this study was analyzed. The phytochemical compounds: Flavoniod,
Alkaliod, Glycoside, Terpine, Saponin, Taninewere obtained by according to suitable extraction protocols. Using HPLC technique,
some polyphenolic compounds were determined. It is noted the presence of Quercitine, Luteolin and Apegeninein macerated and
soxhlet extracts of leaves, while absence of Catechin and Rutine. Macerated and soxhlet extracts of twigs has been observed the
presence of both Quercitine and Catechin.
The inhibitory effect of Rosmarinus officinalis L. extracts on PAO I activity was studied. The macerated extract of twigs with 1
mg/ml and Soxhlet extract of leaves with 4 mg/ml showed 100% inhibition. Lineweaver-Burk plots show a non competitive
inhibition of PAO by using pure Quercitine. Km value was 35.71 mM, which represent K’m value with inhibitor. V max value is
variable from 0.185 unit/ml without inhibitor to 0.138 and 0.107 unit/ml with 4 and 6 mM of quercitine as inhibitor, respectively.
Accordingly, inhibition constant Ki values were 5 and 7 mM, respectively. Our study is aimed to investigate the polyphenolic
composition of rosemary in Mosul, to reveal the affluence of these compounds and assess the inhibitory effect of its extracts. - Scientific Type
- اكاديمي
- Journal volume
- vol.20,No.2
- Collaboration type
- مشترك
- Publish Date
- October 1, 2020
- Scopus status
- In Scopus
- Scopus index year
- 2 020
- Scopus quarter
- 4
- Scopus citation score
- 0.200000003
- Clarivate status
- Not In Clarivate
- Pub. Med. status
- Not In PubMed
- Author (Publication)
- سارة عبد الاله يونس محمد
- Journal (Publication)
- Biochemical and Cellular Archives
- Publisher (Publication)
- DR. P. R. YADAV
- ISSN
- 0972-5075
- Country (Publication)
-
India
- Country type
- عالمية
- College (Publication)
-
College of Health and Medical Technologies
- Departement (Publication)
-
Department of Radiology
- Media
-
Academic Paper
