The paths toward non-viral CAR-T cell manufacturing: A comprehensive review of state-of-the-art methods · Alnoor Digital Repository

Title: The paths toward non-viral CAR-T cell manufacturing: A comprehensive review of state-of-the-art methods
Abstract: Although CAR-T cell therapy has emerged as a game-changer in cancer immunotherapy several bottlenecks limit its widespread use as a front-line therapy. Current protocols for the production of CAR-T cells rely mainly on the use of lentiviral/retroviral vectors. Nevertheless, according to the safety concerns around the use of viral vectors, there are several regulatory hurdles to their clinical use. Large-scale production of viral vectors under “Current Good Manufacturing Practice” (cGMP) involves rigorous quality control assessments and regulatory requirements that impose exorbitant costs on suppliers and as a result, lead to a significant increase in the cost of treatment. Pursuing an efficient non-viral method for genetic modification of immune cells is a hot topic in cell-based gene therapy. This study aims to investigate the current state-of-the-art in non-viral methods of CAR-T cell manufacturing. In the first part of this study, after reviewing the advantages and disadvantages of the clinical use of viral vectors, different non-viral vectors and the path of their clinical translation are discussed. These vectors include transposons (sleeping beauty, piggyBac, Tol2, and Tc Buster), programmable nucleases (ZFNs, TALENs, and CRISPR/Cas9), mRNA, plasmids, minicircles, and nanoplasmids. Afterward, various methods for efficient delivery of non-viral vectors into the cells are reviewed.
Scientific Type: غير معروف
Link: https://www.sciencedirect.com/science/article/abs/pii/S002432052400273X
Journal volume: Volume 348
Collaboration type: مشترك
Publish Date: May 1, 2024
Participated Universities (Publication): Alnoor University
Scopus status: In Scopus
Scopus index year: 2 024
Scopus quarter: 1
Scopus citation score: 10.80000019
Clarivate status: In Clarivate
Clarivate index year: 2 024
Clarivate impafact: 9.199999809
Pub. Med. status: In PubMed
Author (Publication): اشرف محمد حامد سالم
Journal (Publication): Life Sciences
Publisher (Publication): N/A
ISSN: 0024-3205
Country (Publication): United kingdom
Country type: عالمية
College (Publication): College of Dentistry
Departement (Publication): Department of Dentistry