Effect of diode laser rays in stimulating anthocyanin pigment levels in radish (Raphanus sativus L.) plant tissues · Alnoor Digital Repository

Title: Effect of diode laser rays in stimulating anthocyanin pigment levels in radish (Raphanus sativus L.) plant tissues
Abstract: Plant tissue culture is the method to produce many pigments, such as anthocyanin, regarding the importance of light in plant growth, especially when using laser rays. The present study aimed to establish the effect of laser rays on seed germination, initiation of callus, and the measurement of the amounts of anthocyanin pigment and protein for the growth of radish (Raphanus sativus L.). Sterilizing seeds were cultured on a surface of MS medium, then seedlings stems were exposed to laser rays for 0, 4, 8, and 12 minutes each alone and planted on MS medium supplemented with 0.5 mg/l of NAA and 1.0 mg/l of BA, the total protein of seedlings was estimated from all kinds of seedlings exposed to the rays. The anthocyanin pigment content was estimated and read Spectroscopically at a wavelength of 528 nm. The results refer to the different rates and periods of radish seed germination dependent on the exposure time to the laser rays. They also showed the ability of seedlings to initiate callus from hypocotyl stems, which was the fastest exposed for 12 minutes after 7 days at a rate of 100 %. The seeds which were previously exposed to diode laser rays at different times recorded a superiority in stimulating anthocyanin pigment content, which amounted to 1195.2 µg/g for stems and 333.8 µg/g for leaves over the rest of the treatments and the control treatment. This proved to be a modern method to develop forms of physical stimulation as a bio elicitor for the growth of radish tissue cells.
Scientific Type: غير معروف
Link: https://journals.ansfoundation.org/index.php/jans/article/view/5779
Journal volume: Volume 16, Issue 3
Collaboration type: مشترك
Publish Date: September 19, 2024
Participated Universities (Publication): Alnoor University
Scopus status: In Scopus
Scopus index year: 2 024
Scopus quarter: 4
Scopus citation score: 0.899999976
Clarivate status: Not In Clarivate
Pub. Med. status: Not In PubMed
Author (Publication): صفوان جاسم سلطان خليل
Journal (Publication): Journal of Applied and Natural Science
Publisher (Publication): Applied and Natural Science Foundation
ISSN: 0974-9411
Country (Publication): India
Country type: عالمية
College (Publication): College of Dentistry
Departement (Publication): Department of Dentistry

Media: Academic paper